The utilization of yolk platelets by tissues of Xenopus embryos studied by a safranin staining method.
نویسندگان
چکیده
منابع مشابه
Changes in yolk platelet pH during Xenopus laevis development correlate with yolk utilization. A quantitative confocal microscopy study.
The variations of the pH in Xenopus yolk platelets have been estimated by fluorescence confocal microscopy and computer image processing. For pH measurements in vitellogenic oocytes, the pH-sensitive fluorescent dye, DM-NERF, was coupled to vitellogenin, and the DM-NERF-vitellogenin was taken up by oocytes via receptor-mediated endocytosis. Dual emission ratio measurements of internalized DM-NE...
متن کاملXenopus laevis lectin is localized at several sites in Xenopus oocytes, eggs, and embryos
The endogenous lectin of Xenopus laevis oocytes, unfertilized eggs, and blastula-stage embryos was immunohistochemically localized using a highly specific antiserum. Each tissue was examined with several techniques, including paraformaldehyde or glutaraldehyde fixation, frozen or plastic sections, and immunofluorescence or immunoperoxidase staining. In oocytes and unfertilized eggs, lectin was ...
متن کاملThe mechanism and pattern of yolk consumption provide insight into embryonic nutrition in Xenopus.
Little is known about how metabolism changes during development. For most animal embryos, yolk protein is a principal source of nutrition, particularly of essential amino acids. Within eggs, yolk is stored inside large organelles called yolk platelets (YPs). We have gained insight into embryonic nutrition in the African clawed frog Xenopus laevis by studying YPs. Amphibians follow the ancestral...
متن کاملYolk Utilization in the Notochord of Newt as Studied by Electron Microscopy.
W H E N cells of amphibian embryos differentiate they begin to synthesize the structural proteins characteristic of the cell type, and this is immediately preceded by the beginning of the absorption of the yolk platelets. These events may be studied in cultured cells (Jones & Elsdale, 1963) or in sections of fixed embryos. When amphibian platelets are utilized by the cell, they undergo certain ...
متن کاملHigh-magnification in vivo imaging of Xenopus embryos for cell and developmental biology.
Embryos of the frog Xenopus laevis are an ideal model system for in vivo imaging of dynamic biological processes, from the inner workings of individual cells to the reshaping of tissues during embryogenesis. Their externally developing embryos are more amenable to in vivo analysis than internally developing mammalian embryos, and the large size of the embryos make them particularly suitable for...
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عنوان ژورنال:
- Journal of embryology and experimental morphology
دوره 14 2 شماره
صفحات -
تاریخ انتشار 1965